2011030014
宋予晴Title:
Isolation of Total RNA from Animal Tissues and Gel Electrophoresis
Background:
Objectives:
Master the principle, methods of RNA isolation and detection;
Learn some
related techniques for handling RNA.
Principle:
Each mammalian cell contains about 10pg of RNA, of which 80-85% is ribosomal RNA (
rRNA
) (28S, 18S and 5S
varietes
), 10-15% is made up of smaller species, such as transfer RNA(
tRNA
) and 1-5% is messenger RNA(mRNA).
Most mRNAs have a long tail of
adenine(
A)-conta ...