摘要翻译：
由古DNA提取物生成的单链文库有特定的测序要求。通常与古DNA模板相关的短文库分子在流式细胞退火和随后的簇产生（桥式PCR）过程中表现不同，需要优化负载量以获得最佳和一致的簇密度。3年来，我们在波茨坦大学生物化学与生物学研究所的Illumina NextSeq 500测序平台上进行了单链库的测序。我们在这里报告我们的乐观。对于其他希望在NextSeq500平台上对单链库进行测序的研究人员来说，本文档可能是有用的。它并不取代Illumina提供的优秀文档（下面提供的链接），而是作为特定于单链库的附加信息。还应该详细研究描述库协议的原始论文，并补充这里提供的信息。
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英文标题：
《Sequencing single-stranded libraries on the Illumina NextSeq 500
  platform》
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作者：
Johanna L.A. Paijmans, Sina Baleka, Kirstin Henneberger, Ulrike H.
  Taron, Alexandra Trinks, Michael V. Westbury, Axel Barlow
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最新提交年份：
2017
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分类信息：

一级分类：Quantitative Biology        数量生物学
二级分类：Other Quantitative Biology        其他定量生物学
分类描述：Work in quantitative biology that does not fit into the other q-bio classifications
不适合其他q-bio分类的定量生物学工作
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英文摘要：
  Single-stranded libraries generated from ancient DNA extracts have specific sequencing requirements. The short library molecules typically associated with ancient DNA templates are expected to behave differently during the annealing to the flow cell and subsequent cluster generation (bridge PCR), requiring optimisation of loading amounts to obtain optimal and consistent cluster densities. For the past 3 years, we have carried out sequencing of single-stranded libraries on the Illumina NextSeq 500 sequencing platform at the Institute for Biochemistry and Biology, University of Potsdam. We report our optimisations here. This document may be useful for other researchers wishing to sequence single-stranded libraries on the NextSeq 500 platform. It does not replace the excellent documentation provided by Illumina (links provided below), but rather serves as additional information specific to single-stranded libraries. The original papers describing the library protocol should also be studied in detail, and complement the information presented here.
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PDF链接：
https://arxiv.org/pdf/1711.11004