Isolation and Identification of Inner Mongolia Strain of ALV Subgroup J

YANG Yu-ying~1, YIE Jian-qiang~2, ZHAO Zhen-hua~1, QIN Ai-jian~2, GU Yu-fang~1 (1. Inner Mongolia University of Agriculture, Huhhot 010018, China; 2. Yangzhou University, Yangzhou 225009, China)  

A strain of Avian leukosis virus (ALV-J) subgroup J was isolated from an adult broiler breeder folck, which had a history of myeloid leukosis(ML), in Inner Mongolian. Moreover, another strain of ALV-J was isolated from a ML case in Shandong. The two isolates were positive reaction in polymerase chain reactions with two pairs of primers specific for ALV-J and gave antigenically strong reaction in the indirect fluorescence antibody assay(IFA)with ALV-J specific monoclonal antibody JE9. The sequences of the 2.2kb PCR products of the two strains had 96.9% homology, and had 94.2%～95.4% homology to strain SD9901 and YZ9901 ALV-J.
【Key Words】： Avian leukosis virus subgroup J(ALV-J) Isolation and identification
【CateGory Index】： S852.65