analyzing dbGaP-protected data

1473

收藏 2016-08-11

Rail-dbGaP: analyzing dbGaP-protected data in the cloud with Amazon Elastic MapReduce8h
[/url][url=]

由 Nellore, A., Wilks, C., Hansen, K. D., Leek, J. T., Langmead, B.[url=][/url] 通过 Bioinformatics - current issue[url=][/url]

Motivation: Public archives contain thousands of trillions of bases of valuable sequencing data. More than 40% of the Sequence Read Archive is human data protected by provisions such as dbGaP. To analyse dbGaP-protected data, researchers must typically work with IT administrators and signing officials to ensure all levels of security are implemented at their institution. This is a major obstacle, impeding reproducibility and reducing the utility of archived data.
Results: We present a protocol and software tool for analyzing protected data in a commercial cloud. The protocol, Rail-dbGaP, is applicable to any tool running on Amazon Web Services Elastic MapReduce. The tool, Rail-RNA v0.2, is a spliced aligner for RNA-seq data, which we demonstrate by running on 9662 samples from the dbGaP-protected GTEx consortium dataset. The Rail-dbGaP protocol makes explicit for the first time the steps an investigator must take to develop Elastic MapReduce pipelines that analyse dbGaP-protected data in a manner compliant with NIH guidelines. Rail-RNA automates implementation of the protocol, making it easy for typical biomedical investigators to study protected RNA-seq data, regardless of their local IT resources or expertise.
Availability and Implementation: Rail-RNA is available from http://rail.bio. Technical details on the Rail-dbGaP protocol as well as an implementation walkthrough are available at [url]https://github.com/nellore/rail-dbgap. Detailed instructions on running Rail-RNA on dbGaP-protected data using Amazon Web Services are available at http://docs.rail.bio/dbgap/[/url].
Contacts: anellore@gmail.com or langmea@cs.jhu.edu
Supplementary information: Supplementary data are available at Bioinformatics online.