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2022-03-04
摘要翻译:
本文介绍了正电子湮没寿命谱(PALS)和简单微生物&酿酒酵母的显微研究结果。在冻干酵母和水相酵母中,正电子素(o-Ps)的寿命分别从2.4ns变化到2.9ns(更长寿命成分)。同时还对酵母的吸湿性进行了检测,从而检验了作为细胞主要成分的水是如何影响PALS参数的,从而发现干酵母的o-Ps寿命从1.2到1.4ns(寿命较短的成分)变化。在10小时以下发现足够的时间使细胞水化。在液态水存在下,观察到酵母在分子尺度上重组的迹象。利用倒置显微镜(IM)和环境扫描电镜(ESEM)方法获得了冻干、干、湿酵母的显微图像。结果在ESEM图像中观察到细胞膜表面的可见变化。
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英文标题:
《Studies of unicellular micro-organisms Saccharomyces cerevisiae by means
  of Positron Annihilation Lifetime Spectroscopy》
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作者:
E. Kubicz, B. Jasi\'nska, B. Zgardzi\'nska, T. Bednarski, P.
  Bia{\l}as, E. Czerwi\'nski, A. Gajos, M. Gorgol, D. Kami\'nska, {\L}.
  Kap{\l}on, A. Kochanowski, G. Korcyl, P. Kowalski, T. Kozik, W. Krzemie\'n,
  S. Nied\'zwiecki, M. Pa{\l}ka, L. Raczy\'nski, Z. Rajfur, Z. Rudy, O. Rundel,
  N.G. Sharma, M. Silarski, A. S{\l}omski, A. Strzelecki, A. Wieczorek, W.
  Wi\'slicki, M. Zieli\'nski, P. Moskal
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最新提交年份:
2015
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分类信息:

一级分类:Quantitative Biology        数量生物学
二级分类:Other Quantitative Biology        其他定量生物学
分类描述:Work in quantitative biology that does not fit into the other q-bio classifications
不适合其他q-bio分类的定量生物学工作
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一级分类:Physics        物理学
二级分类:Instrumentation and Detectors        仪器仪表和探测器
分类描述:Instrumentation and Detectors for research in natural science, including optical, molecular, atomic, nuclear and particle physics instrumentation and the associated electronics, services, infrastructure and control equipment.
用于自然科学研究的仪器和探测器,包括光学、分子、原子、核和粒子物理仪器和相关的电子学、服务、基础设施和控制设备。
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英文摘要:
  Results of Positron Annihilation Lifetime Spectroscopy (PALS) and microscopic studies on simple microorganisms: brewing yeasts are presented. Lifetime of ortho - positronium (o-Ps) were found to change from 2.4 to 2.9 ns (longer lived component) for lyophilised and aqueous yeasts, respectively. Also hygroscopicity of yeasts in time was examined, allowing to check how water - the main component of the cell - affects PALS parameters, thus lifetime of o-Ps were found to change from 1.2 to 1.4 ns (shorter lived component) for the dried yeasts. The time sufficient to hydrate the cells was found below 10 hours. In the presence of liquid water an indication of reorganization of yeast in the molecular scale was observed.   Microscopic images of the lyophilised, dried and wet yeasts with best possible resolution were obtained using Inverted Microscopy (IM) and Environmental Scanning Electron Microscopy (ESEM) methods. As a result visible changes to the surface of the cell membrane were observed in ESEM images.
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PDF链接:
https://arxiv.org/pdf/1509.00411
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