摘要翻译：
传统上，阿尔泰、椴树和番石榴被用来治疗呼吸道感染症状。山楂花、山楂叶、瓜蒌叶用于治疗咳嗽、咽喉肿痛、卡他、口腔和咽部黏膜刺激。因此，本研究旨在检测这些植物的抗菌和抗生物膜作用，单独以及联合，作为一种抗呼吸道感染病原体的配方。检测的病原菌为产超广谱β-内酰胺酶的大肠杆菌(ESBL)、产β-内酰胺酶的大肠杆菌(BL)、产β-内酰胺酶的肺炎克雷伯菌(BL)、产β-内酰胺酶的铜绿假单胞菌(BL)、阴沟肠杆菌和产β-内酰胺酶的金黄色葡萄球菌(BL)。试验植物用乙醇提取，然后用不同极性的溶剂（己烷、乙酸乙酯和水）进行分级。采用纸片扩散法和微量稀释法（最低抑菌浓度）评价抗菌活性，结晶紫法测定生物膜活性。结果表明，铁皮草和铁皮草的提取物及部分均表现出较弱的抑菌活性，MIC值在6.25～12.5mg/ml之间。瓜蒌具有中等的抑菌活性，MIC值为6.25～1.56mg/ml。这些植物提取物与各部分按等比例组合，具有较强的抑菌活性(MIC50为0.2mg/ml)，MIC值范围为6.25～0.8mg/ml)和抗生物膜活性(MBIC50为0.2mg/ml)。因此，这项研究提供了一个有价值的科学知识来支持组合而不是单独使用植物。
---
英文标题：
《Antibacterial and antibiofilm activities of a traditional herbal formula
  against respiratory infection causing bacteria》
---
作者：
H.A Qaralleh, M.O. Al-Limoun, A. Khlaifat, K.M. Khleifat, N.
  Al-Tawarah, K.Y. Alsharafa, H.A. Abu-Harirah
---
最新提交年份：
2021
---
分类信息：

一级分类：Quantitative Biology        数量生物学
二级分类：Other Quantitative Biology        其他定量生物学
分类描述：Work in quantitative biology that does not fit into the other q-bio classifications
不适合其他q-bio分类的定量生物学工作
--

---
英文摘要：
  The plants, Althaea officinalis, Tilia cordata and Psidium guaja have been used traditionally to treat respiratory infection symptoms. Flowers of A. officinalis and leaves of T. cordata and P. guaja have been used to treat cough, sore throat, catarrh, oral and pharyngeal mucosa irritation. Therefore, this study was designed to examine the antibacterial and antibiofilm effects of these plants individually as well as in combination, as a formula against respiratory infections causing pathogens. The tested pathogens were Extended Spectrum Beta-Lactamase producing Escherichia coli (ESBL), Beta-Lactamase producing Escherichia coli (BL), Beta-Lactamase producing Klebsiella pneumoniae (BL), Beta-Lactamase producing Pseudomonas aeruginosa (BL), Enterobacter cloacae, and Beta-Lactamase producing Staphylococcus aureus (BL). The tested plants were extracted using ethanol and then fractionated using different polarity solvents (hexane, ethyl acetate and water). Disc diffusion and microdilution (Minimum Inhibitory Concentration) methods were used to evaluate the antibacterial activity while the antibiofilm activity was tested using crystal violet assay. The results showed that A. officinalis and T. cordata extracts and fractions exhibited weak antibacterial activity having MIC values ranged from 6.25 to 12.5 mg/mL. P. guaja exhibited moderate antibacterial activity with MIC values ranged from 6.25 to 1.56 mg/mL. Combination between these plants extracts and fractions in equal proportion provides stronger antibacterial (with MIC values ranged from 6.25 to 0.8 mg/mL) and antibiofilm activities (MBIC50 was 0.2 mg/mL). Therefore, this study provides a valuable scientific knowledge to support the use of plants in combination rather than individually.
---
PDF链接：
https://arxiv.org/pdf/2102.04301