There was a time when to amplify DNA,从前你要扩增DNA， You had to grow tons and tons of tiny cells.总有培养大批细胞的重任要你背。 Then along came a guy named Dr. Kary Mullis,这时有个家伙叫Kary Mullis博士的， Said you can amplify in vitro just as well.他钻出来说体外合成也一样OK！ Just mix your template with a buffer and some primers,只要把你的模板混上缓冲液，再加些引物， Nucleotides and polymerases, too.还有核苷和聚合酶。 Denaturing, annealing, and extending.变性，退火，和延伸。 Well it’s amazing what heating and cooling and heating will do.加热~冷却~加热~太神奇了！ PCR, when you need to d ...