摘要翻译:
这项调查的主要目的是阐明以前从患者中分离出的铜绿假单胞菌铜摄取水平的信息,这些铜绿假单胞菌使用革兰氏阴性产气杆菌和革兰氏阳性苏云金芽孢杆菌作为铜摄取测量的对照。Cu摄取数据显示,铜绿假单胞菌对Cu的摄取量最大。金属吸收依赖于生物吸附剂的类型,对所测铜的化学形态具有不同的积累亲和力。在指数生长期收获的细胞对Cu的摄取略高于固定期,说明细胞对Cu的摄取是代谢依赖的过程。氯化铜和硫酸铜对三属植物亲和力的增加顺序基本不变。在硝酸铜的作用下,气绿假单胞菌对铜的吸收行为与其他两种化学形式的结果没有明显的变化,而在产气E.和苏云金B.中,气绿假单胞菌对铜的吸收行为不同。在B.Thuengenuce中,铜的吸收速率随Cu初始浓度的增加而增加,与其它条件相比,铜的吸收速率一直在320 ppm以上。在含有硝酸铜的肠杆菌中,也显示了不同的巴哈维菌,其铜摄取量急剧下降到320 ppm以上。这些结果表明铜绿假单胞菌吸收铜的机制与其它供试菌不同。
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英文标题:
《Effect of Copper Chemical Form on The Growth of Pseudomonas aeruginosa
  Isolated from Burned Patients and on Its Cu Uptake》
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作者:
Amjad Al Tarawneh, Haitham Qaralleh, Muhamad O. Al-limoun, Khaled M.
  Khleifat
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最新提交年份:
2019
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分类信息:
一级分类:Quantitative Biology        数量生物学
二级分类:Other Quantitative Biology        其他定量生物学
分类描述:Work in quantitative biology that does not fit into the other q-bio classifications
不适合其他q-bio分类的定量生物学工作
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英文摘要:
  The main object of this investigation was to shed light on the information on the level of Cu uptake by Pseudomonas aeruginosa that has been previously isolated from patients using Gram-negative E. aerogenes ans a Gram-positive Bacillus thuringiensis as a control for Cu uptake measurements. Cu uptake data showed that maximum Cu uptake was obtained by the Pseudomonas aeruginosa. The metal uptake was dependent on the type of biosorbent with different accumulation affinities toward the tested chemical form of the copper. Cells harvested at exponential growth phase showed slightly higher Cu uptake than at stationary phase, which reflect that the Cu uptake is metabolism dependent-process. The increasing order of affinity of the cupric chloride and cupric sulphate towards the three genera were almost constant. However, where cupric nitrate was used, the copper uptake behavior in Pseudomonas aeroginosa was not changed from those results with the other two chemical forms, whereas with E. aerogenes & B. thuringiensis, different Cu uptake behaviors were observed. In B. thuengenesis, the copper uptake rate as a function of initial Cu concentration were shown to increase with increasing the initial concentration of Cu, constantly above 320 ppm as compared with others. In Enterobacter with cupric nitrate, different bahavior was also shown in which the copper uptake decline sharply beyond the 320 ppm. These results reflect that the Cu uptake in P. aeruginosa is with different mechanisms as compared with other tested bacteria. 
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PDF链接:
https://arxiv.org/pdf/1911.10362