摘要翻译：
光显微镜允许以亚微米分辨率观察细胞特征和物体。因此，光显微镜在生命科学中起着基础作用已经有一百多年了。在大量生产的电子产品和硬件、公开共享的文档和建筑说明、开放源码软件、快速原型和3D打印的广泛使用以及相关贡献者和用户的热情的推动下，开放显微镜的概念获得了令人难以置信的势头，为不断扩大的用户群带来了新的复杂工具。在这里，我们将首先讨论开放科学和开放显微镜背后的思想，然后突出开放显微镜的最新项目和发展。我们认为，面向广大用户群体甚至非专家的设计良好的开放硬件和软件解决方案的可用性，将越来越有助于应对尖端成像技术日益复杂的问题。然后我们将广泛讨论开放显微镜目前和未来的挑战。
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英文标题：
《Open microscopy in the life sciences: Quo Vadis?》
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作者：
Johannes Hohlbein, Benedict Diederich, Barbora Marsikova, Emmanuel G.
  Reynaud, Seamus Holden, Wiebke Jahr, Robert Haase, and Kirti Prakash
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最新提交年份：
2021
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分类信息：

一级分类：Quantitative Biology        数量生物学
二级分类：Other Quantitative Biology        其他定量生物学
分类描述：Work in quantitative biology that does not fit into the other q-bio classifications
不适合其他q-bio分类的定量生物学工作
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一级分类：Physics        物理学
二级分类：Biological Physics        生物物理学
分类描述：Molecular biophysics, cellular biophysics, neurological biophysics, membrane biophysics, single-molecule biophysics, ecological biophysics, quantum phenomena in biological systems (quantum biophysics), theoretical biophysics, molecular dynamics/modeling and simulation, game theory, biomechanics, bioinformatics, microorganisms, virology, evolution, biophysical methods.
分子生物物理、细胞生物物理、神经生物物理、膜生物物理、单分子生物物理、生态生物物理、生物系统中的量子现象（量子生物物理）、理论生物物理、分子动力学/建模与模拟、博弈论、生物力学、生物信息学、微生物、病毒学、进化论、生物物理方法。
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一级分类：Physics        物理学
二级分类：Data Analysis, Statistics and Probability        数据分析、统计与概率
分类描述：Methods, software and hardware for physics data analysis: data processing and storage; measurement methodology; statistical and mathematical aspects such as parametrization and uncertainties.
物理数据分析的方法、软硬件：数据处理与存储；测量方法；统计和数学方面，如参数化和不确定性。
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一级分类：Physics        物理学
二级分类：Instrumentation and Detectors        仪器仪表和探测器
分类描述：Instrumentation and Detectors for research in natural science, including optical, molecular, atomic, nuclear and particle physics instrumentation and the associated electronics, services, infrastructure and control equipment.
用于自然科学研究的仪器和探测器，包括光学、分子、原子、核和粒子物理仪器和相关的电子学、服务、基础设施和控制设备。
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一级分类：Physics        物理学
二级分类：Optics        光学
分类描述：Adaptive optics. Astronomical optics. Atmospheric optics. Biomedical optics. Cardinal points. Collimation. Doppler effect. Fiber optics. Fourier optics. Geometrical optics (Gradient index optics. Holography. Infrared optics. Integrated optics. Laser applications. Laser optical systems. Lasers. Light amplification. Light diffraction. Luminescence. Microoptics. Nano optics. Ocean optics. Optical computing. Optical devices. Optical imaging. Optical materials. Optical metrology. Optical microscopy. Optical properties. Optical signal processing. Optical testing techniques. Optical wave propagation. Paraxial optics. Photoabsorption. Photoexcitations. Physical optics. Physiological optics. Quantum optics. Segmented optics. Spectra. Statistical optics. Surface optics. Ultrafast optics. Wave optics. X-ray optics.
自适应光学。天文光学。大气光学。生物医学光学。基本点。准直。多普勒效应。纤维光学。傅里叶光学。几何光学（梯度折射率光学、全息术、红外光学、集成光学、激光应用、激光光学系统、激光、光放大、光衍射、发光、微光学、纳米光学、海洋光学、光学计算、光学器件、光学成像、光学材料、光学计量学、光学显微镜、光学特性、光学信号处理、光学测试技术、光波传播、傍轴光学、光吸收、光激发、物理光学、生理光学、量子光学、分段光学、光谱、统计光学、表面光学、超快光学、波动光学、X射线光学。
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英文摘要：
  Light microscopy allows observing cellular features and objects with sub-micrometer resolution. As such, light microscopy has been playing a fundamental role in the life sciences for more than a hundred years. Fueled by the availability of mass-produced electronics and hardware, publicly shared documentation and building instructions, open-source software, wide access to rapid prototyping and 3D printing, and the enthusiasm of contributors and users involved, the concept of open microscopy has been gaining incredible momentum, bringing new sophisticated tools to an expanding user base. Here, we will first discuss the ideas behind open science and open microscopy before highlighting recent projects and developments in open microscopy. We argue that the availability of well-designed open hardware and software solutions targeting broad user groups or even non-experts, will increasingly be relevant to cope with the increasing complexity of cutting-edge imaging technologies. We will then extensively discuss the current and future challenges of open microscopy.
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PDF链接：
https://arxiv.org/pdf/2110.13951