摘要翻译:
最近,在数值研究的帮助下,荧光相关光谱在可变观察区可以揭示一个半透性屏障网格的存在,阻碍了标记颗粒(如质膜组分)的二维扩散。我们提出了一个完整的理论来证实和解释这些发现。它能够可靠、定量地利用实验数据,从中提取亚波长网格尺寸。详细讨论了实验中必须进行荧光相关光谱的时间尺度。
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英文标题:
《Theory of fluorescence correlation spectroscopy at variable observation
area for two-dimensional diffusion on a meshgrid》
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作者:
Nicolas Destainville
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最新提交年份:
2007
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分类信息:
一级分类:Physics 物理学
二级分类:Statistical Mechanics 统计力学
分类描述:Phase transitions, thermodynamics, field theory, non-equilibrium phenomena, renormalization group and scaling, integrable models, turbulence
相变,热力学,场论,非平衡现象,重整化群和标度,可积模型,湍流
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一级分类:Quantitative Biology 数量生物学
二级分类:Subcellular Processes 亚细胞过程
分类描述:Assembly and control of subcellular structures (channels, organelles, cytoskeletons, capsules, etc.); molecular motors, transport, subcellular localization; mitosis and meiosis
亚细胞结构(通道、细胞器、细胞骨架、囊膜等)的组装和控制;分子马达;转运;亚细胞定位;有丝分裂和减数分裂
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英文摘要:
It has recently been proposed, with the help of numerical investigations, that fluorescence correlation spectroscopy at variable observation area can reveal the existence of a meshgrid of semi-permeable barriers hindering the two-dimensional diffusion of tagged particles, such as plasmic membrane constituents. We present a complete theory confirming and accounting for these findings. It enables a reliable, quantitative exploitation of experimental data from which the sub-wavelength mesh size can be extracted. Time scales at which fluorescence correlation spectroscopy must be performed experimentally are discussed in detail.
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PDF链接:
https://arxiv.org/pdf/711.4699