摘要翻译：
除了细胞间的化学和机械相互作用外，细胞产生的电磁场被认为是细胞间通信的另一种信号形式。本研究的目的是评价电磁效应对脂肪来源干细胞（ADSCs）存活率的影响，而不是共培养。本研究采用酶法从人脂肪组织中分离干细胞，并在单层培养中进行增殖。每孔培养5.(10^4)个脂肪干细胞。第一行（4孔）以ADSCs为诱导细胞，在DMEM1中加入10 ng/ml成纤维细胞生长因子(FGF)培养。在相邻行和最后一行，无FGF作为检测细胞培养ADSCs。第3天和第5天检测细胞的活力。此外，在相同条件下培养ADSCs，但诱导细胞分别置于紫外滤光管和石英管中各一次，观察细胞间是否存在电磁相互作用。诱导细胞在第5天使相邻行细胞明显增殖（P值<0.01)。而紫外滤光管和石英管均能显著降低诱导细胞对邻近细胞的影响。因此，我们可以检测到脂肪干细胞（ADSCs）之间的远距离细胞相互作用(DCI)，但这不是电磁信号。我们的研究结果表明，ADSCs通过挥发性信号传递相互影响，这是一种化学的远距离细胞相互作用(CDCI)。
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英文标题：
《Experimental Investigation of Distant Cellular Interaction among Adipose
  Derived Stem-Cells》
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作者：
B. Hashemibeni, M Sadeghian, M Aliakbari, Z Alinasab, M. Akbari, V.
  Salari
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最新提交年份：
2014
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分类信息：

一级分类：Quantitative Biology        数量生物学
二级分类：Other Quantitative Biology        其他定量生物学
分类描述：Work in quantitative biology that does not fit into the other q-bio classifications
不适合其他q-bio分类的定量生物学工作
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英文摘要：
  In addition to chemical and mechanical interactions between cells electromagnetic field produced by cells has been considered as another form of signaling for cell-cell communication. The aim of this study is evaluation of electromagnetic effects on viability of Adipose-derived stem cells (ADSCs) without co-culturing. In this study, stem cells were isolated from human adipose tissue enzymatically and proliferated in monolayer culture. Then, 5.(10^4) adipose-derived stem cells were cultured in each well of the test plate. In the first row (4 wells), ADSCs as inducer cells were cultured in DMEM1 with 10 ng/ml Fibroblast growth factor (FGF). In adjacent and the last rows, ADSCs were cultured without FGF (as detector cells). After the three and five days the viability of cells were evaluated. Moreover, ADSCs were cultured in the same conditions but the inducer cells were placed once in the UV-filter tube and once in the quartz tube to see whether there is electromagnetic interaction among cells. Inducer cells caused significant cell proliferation in adjacent row cells (p- value<0.01) in the fifth day. However, using the UV-filter tube and quartz tube both reduced the effect of inducer cells on adjacent cells significantly. As a conclusion, we could detect distant cellular interaction (DCI) among adipose derived stem cells (ADSCs), but it was not electromagnetic signaling. Our results show that ADSCs affect each other via volatile signaling as a chemical distant cellular interaction (CDCI).
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PDF链接：
https://arxiv.org/pdf/1411.1498