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2022-03-03
摘要翻译:
产胞外碱性蛋白酶的菌株是从土壤中分离出来的一株,经鉴定为粉状链霉菌MTCC8374。对深层发酵的研究表明,在固定期的早期是产酶量的最大值。最适pH值为9.0%,最适接种量为3%,最适温度为33℃。在碳源中,0.3peccer淀粉产量最高,其次是麦芽糖、木糖和果糖。报道了1.0%酪蛋白的高产率,其次是豆粕、酵母抽提物和麦芽抽提物。在此基础上,以0.5%、1.0%和1.5%的NaCl为优化条件,其中1%NaCl的蛋白酶含量最高。该菌株的蛋白酶谱显示了其作为工业应用的良好来源的潜力。
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英文标题:
《Optimization of Production Protocol of Alkaline Protease by Streptomyces
  pulvereceus》
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作者:
D. Jayasree, T.D. Sandhya Kumari, P.B. Kavi Kishor, M. Vijaya Lakshmi,
  M.Lakshmi Narasu
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最新提交年份:
2010
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分类信息:

一级分类:Quantitative Biology        数量生物学
二级分类:Other Quantitative Biology        其他定量生物学
分类描述:Work in quantitative biology that does not fit into the other q-bio classifications
不适合其他q-bio分类的定量生物学工作
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英文摘要:
  Extra cellular alkaline protease producing species is an isolate from soil which was characterized and identified as Streptomyces pulvereceus MTCC 8374. Studies on submerged fermentation revealed that maximum level of enzyme production was during early stationary phase. Optimum pH, inoculum and temperature were 9.0, 3 percent and 33 C respectively. Among carbon sources 0.3 peccent starch gave a maximum production followed by maltose, xylose and fructose. High yield of protease production was reported with 1.0 percent casein followed by soybean meal, yeast extract and malt extract. Further, it was optimized with 0.5 percent, 1.0 percentand 1.5 percent of NaCl among which 1 percent NaCl resulted in maximum level of protease. The protease profile of the isolate shows its potential as a good source for industrial application.
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PDF链接:
https://arxiv.org/pdf/1002.0048
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