摘要翻译：
我们研究了Using的带电示踪剂的单向通量和通量比，特别是受Hodgkin和Keynes关于通量比与通道结构关系的精辟建议的推动。我们的研究是基于对离子在膜通道中流动的准一维泊松-能斯特-普朗克模型的分析。这类模型包括泊松方程，它从电荷中确定电势，在这个意义上是一致的。USSING的流量比一般依赖于离子流中涉及的所有物理参数，特别是体积条件和通道结构。离子通道实验的某些设置导致通量比是普遍的，因为它们的值依赖于体条件而不依赖于通道结构；其他设置导致流量比是特定的，因为它们的值也依赖于通道结构。通用磁通比在某些情况下比特定磁通比更好地服务于某些目的，而在其他情况下则更差。我们重点讨论了示踪通量测量的两种处理方法，它们作为离子通道重要性质的估计器。第一个估计器根据其示踪剂通量的测量确定主要离子物种的通量。我们的分析提出了一个更好的实验设计，使示踪剂通量和主离子通量的通量比具有普遍性。示踪剂通量的第二个处理涉及通量的比率和试图确定通道结构某些性质的实验装置。我们分析了两种广泛使用的估算通量比的实验设计，表明最广泛使用的方法依赖于永久电荷的空间分布，因此该通量比是特定的，从而允许估算永久电荷的某些性质，即使在理想的离子溶液中也是如此。...
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英文标题：
《Flux ratios and channel structures》
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作者：
Shuguan Ji, Bob Eisenberg, Weishi Liu
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最新提交年份：
2016
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分类信息：

一级分类：Quantitative Biology        数量生物学
二级分类：Other Quantitative Biology        其他定量生物学
分类描述：Work in quantitative biology that does not fit into the other q-bio classifications
不适合其他q-bio分类的定量生物学工作
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英文摘要：
  We investigate Ussing's unidirectional fluxes and flux ratios of charged tracers motivated particularly by the insightful proposal of Hodgkin and Keynes on a relation between flux ratios and channel structure. Our study is based on analysis of quasi-one-dimensional Poisson-Nernst-Planck type models for ionic flows through membrane channels. This class of models includes the Poisson equation that determines the electrical potential from the charges present and is in that sense consistent. Ussing's flux ratios generally depend on all physical parameters involved in ionic flows, particularly, on bulk conditions and channel structures. Certain setups of ion channel experiments result in flux ratios that are universal in the sense that their values depend on bulk conditions but not on channel structures; other setups lead to flux ratios that are specific in the sense that their values depend on channel structures too. Universal flux ratios could serve some purposes better than specific flux ratios in some circumstances and worse in other circumstances. We focus on two treatments of tracer flux measurements that serve as estimators of important properties of ion channels. The first estimator determines the flux of the main ion species from measurements of the flux of its tracer. Our analysis suggests a better experimental design so that the flux ratio of the tracer flux and the main ion flux is universal. The second treatment of tracer fluxes concerns ratios of fluxes and experimental setups that try to determine some properties of channel structure. We analyze the two widely used experimental designs of estimating flux ratios and show that the most widely used method depends on the spatial distribution of permanent charge so this flux ratio is specific and thus allows estimation of (some of) the properties of that permanent charge, even with ideal ionic solutions. ...
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PDF链接：
https://arxiv.org/pdf/1612.08742